|
KMID : 1161520070110020199
|
|
Animal Cells and Systems
2007 Volume.11 No. 2 p.199 ~ p.204
|
|
|
Presynatic expression of HCN channel subunits in cerebellar basket cells
|
|
Yi Jee-Hyun
Park Kyung-Joon
Kang Shin-Jung
Shin Ki-Soon
|
|
|
Abstract
|
|
|
|
HCN (hyperpolarization?activated cyclic nucleotide?gated) channels, whose gene family consists of four subunits (HCN 1?4), mediate depolarizing cation currents and contribute to controlling neuronal excitability. In the present study, immunohistochemical and electrophysiological approaches were used to elucidate the role of HCN channels in the cerebellum. Immunohistochemical labeling for HCN1 and HCN2 channels revealed localized expression of both channels at pinceau, the specialized structure of presynaptic axon terminals of basket cells. To determine the functional role of the presynaptic HCN channels, spontaneous inhibitory postsynaptic currents (IPSCs) were recorded from Purkinje cells, the main synaptic targets of basket cells in the cerebellum. While activation of HCN channels by 8?bromo?cAMP increased amplitude of spontaneous IPSCs, blockade of the activated HCN channels by subsequent ZD7288 application reduced the amplitude of spontaneous IPSCs to the level far below the control. Our results imply that modulation of HCN1 and HCN2 channels in presynaptic terminals of basket cells regulates neurotransmitter release, thereby controlling the excitability of Purkinje cells.
|
|
|
KEYWORD
|
|
|
HCN channels, IPSC, Basket cells, ZD7288, Cerebellum
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|